小鼠白色念珠菌逆行性肾盂肾炎的免疫功能测定

The determination of immune functions of mouse experimental pyelonephritis infected with c. albicans

本实验建立了小鼠白色念珠菌肾盂肾炎模型,并研究了其免疫功能。实验显示,感染30天时,模型动物脾细胞对ConA 刺激的T细胞增殖反应、ConA诱导的IL—2活性均增强。对LPS诱导的B细胞增殖反应增强,以15天最为显著。对SRBC初次抗体应答,在15天、30天时均增强。对白念可溶性抗原的DTH反应性,在15天时升高直到30天时明显。在感染15天时血清特异性凝集效价迅速增高,直至30天仍保持一个高值。

A pyelonephritis model of mouse is made by c. albicans infection. The determination of im-mune functions in model show that, both proliferation response of T cell to ConA and IL-2 activ-iting of spleenocytes of model animal to inductionwith ConA increased at 30 days after injected.The proliferation response of B cell to stimulation with LPS inhanced from 15 through 30 days,primary Ab response of model animal to SRBC stimulation inhanced between 15-30 days afterimmunization. DTH response to stimulation with c. albicans protein Ag raised since 15 days andup to peak at 30 days. Agglutination titer of serum raised up 15 days, and remained at higherlevel untill 30 days.