摘要
通过分析5一溴脱氧尿嘧啶核苷(5-bromodeoxyuridine,BrdU)掺入后的第2周期细胞染色体,同步评估了受试化合物秋水仙素(colchicine,COL)及昆明山海棠水抽提物(Tripteryguimhypoglaucum(Leul)Hutch,THH)在小鼠骨髓细胞中的有丝分裂阻滞、染色体异常分离及姐妹染色单体互换(sisterchromatidexchange,SCE)诱发效应。COL及THH均具有不同程度的有丝分裂阻滞效应,且显著诱发非整倍体(2n=41-43)及多倍体的产生。THH尚显著诱发小鼠骨髓细胞SCE。结果提示:THH具有与COL类似的哺乳动物体细胞非整倍体/多倍体诱发效应;此外,THH尚含有某些基因毒性成分.实验还证实,BrdU标记法检检测哺乳动物体细胞染色体异常分离是可行的。
Abstract The effects of mitotic block ,
chromosome malsegregation and sister chromatidexchange(SCE)induction were
simultaneously investigated、with Bromodeoxyuridine(Br-dU) labeled mouse bone [marrow
cells at their second generation after a single i.p. treat-ment(COL ) and Triperygium
hypoglaucum (Leul)Hutch(THH). Significant delay ofcell cycle were found after amimals were
treated with both COL and THH. COL and THHalso induced aneuploidy significantly(2n = 41~
43 ) and polypoloidy in second mitosis.Asignificant increased SCE over the control frequency
was oherserved after 5g/kg and 10g/kg of THH treatment.The result indicated that THH share
the induction effects of aneu-ploidy and polyploidy as COL. Besides this, THH also possess
genotoxicity. The approachto estimate in vivo mitotic malsegregation in mammalian somatic
cells based Brdu incorpo-ration is vaiid.
出处
《癌变.畸变.突变》
CAS
CSCD
1994年第4期1-5,共5页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金
云南省科委基金