酸性磷酸酶在萌发大豆种子中子叶细胞壁上的分布

LOCALIZATION OF THE ACID PHOSPHATASE ACTIVITY ON THE PORTIONS OF COTYLEDON CELL WALL

本文应用光镜、电镜酸性磷酸酶的细胞化学定位法，对大豆种子萌发过程中酸性磷酸酶在子叶细胞壁上的分布进行了详细的观察与分析。大豆种子吸水膨胀１天时，子叶细胞的胞间层首先表现出酸性磷酸酶的活性；播种１－２天后，除胞间层外，胞间隙也表现了酸性磷酸酶的活性；播种４－６天期间，酸性磷酸酶不仅在胞间层、胞间隙处表现出活性，而且在细胞壁及质膜上也表现出较强的活性。根据本文的观察结果我们推测，萌发的大豆子叶细胞壁上分布的酸性磷酸酶，是在种子形成过程中合成并积累于此处的。

Acid phosphatase activity was located by cytochemical techniquse on the portions of cotyledon cell wall of germinating soybean,When soybean seeds started to germinate for the lst day,the acid phosphatase activity first appeared in regions of the middle lamella between two cotyledon cells.At 1- 2 days affer planting,the activity was present in the corners among the cells as well as the middle lamalla.At 4 - 6 days after planting,the enzyme activity showed also in the portions of cell wall and on plasmalamma. Based on the above results, we draw a comclution that the acid phosphatase activity on the portions of the cell wall of germinating cotyledons was synthesized and deposited when the seeds were doveloping.