摘要
利用发光测量法,研究了中药复方、植物分泌液(SL)和植物分泌液复方(SLIV)对Fe2+-亚麻油酸-BSA(牛血清白蛋白)和Fe2+-脑勺浆两个离体体系产生的脂质过氧化(LPO)有抑制作用,抑制程度与用药浓度有关.将中药复方应用于辐照模型,结果表明7Gy的γ射线辐照后小白鼠全血硒谷胱甘肽过氧化物酶(SeGSH-Px)活性降低,恢复缓慢,脑组织脂质过氧化加剧,而事先喂饲中药复方能显著提高SeGSH-Px水平,加速其活力恢复,增强脑组织抗氧化能力;将SL和SLIV应用于脑缺血重灌模型,结果显示沙土鼠脑缺血重灌后全血SeGSH-Px活力下降,血浆MDA上升,SeGSH-Px/MDA值降低,脑组织抗氧化能力下降,而事先喂饲SL、SLIV上述结果正相反,能明显提高机体的抗LPO能力.
n this paper, the capabilities of CHM,SL and SLIV to inhibit lipid peroxidation (LPO) in vitro and in vivo have been studied. In vitro,the concentration-dependent decreases of ultraweak chemiluninescence were observed when CHM, SL and SLIV were added to both Fe2+-lindenic Acid-BAS and Fe2+-Brain Homogenate system respectively.In vivo, CHM may contribute to the reinforcement and recovery of antiperoxidation abilities of γ-ray-treated mice by increasing the activity of SeGSH-Px. SL and SLIV can protect ischemic-reperfused Mongolian Gerbil against LPO injuries by enhancing the value of SeGSH-Px/MDA. CHM,SL and SLIV also can protect brain tissue against LPO in vivo, shown as lower level of brain homogenate natural or induced chemiluminescence.
出处
《发光学报》
EI
CAS
CSCD
北大核心
1994年第4期348-353,共6页
Chinese Journal of Luminescence
关键词
脂质过氧化
中药复方
植物分泌液
抑制
CHM(a Chinese herb medicine), SL(a kind of plant secretion), SLIV (SL combined with Snow Lotus), LPO, SeGSH-Px, chemiluminescence,ischemia-reperfusion