胁迫条件下盐藻β-胡萝卜素及其异构体累积的研究──盐度的影响

STUDY ON ACCUMULATION OF β-CAROTENE AND ITS ISOMERS IN DUNALIELLA SALINA UNDER ENVIRONMENTAL STRESS CONDITIONS─SALINITY EFFECT

于1989—1991年，在青岛室外以盐藻为实验材料，利用Johnson改良配方制作培养液，进行盐度（90－240）实验。结果表明，细胞生长的适宜盐度为120；当盐度超过120－240，盐度越高对细胞生长越不利，但越有利于细胞内β－胡萝卜素的累积；适宜于养殖盐藻生产β－胡萝卜素的盐度为240。研究证明，盐藻累积的β－胡萝卜素中至少含6种异构体，盐度增加不利于顺式异构体的累积。为生产防癌和延缓衰老等作用更强的顺式异构体，建议生产性养殖时适当降低盐度到210，以便累积更多的β－胡萝卜素及顺式异构体。

The effect of salinity (S) on cell growth, accumulation of β-carotene and its isomers in Dunaliella salina Teodoresco strain 20 cultured outdoors in modified Johnson's medium of salinity 90-240 was conducted in 1989-1991 in Qingdao. The results showed that the S favourable for growth is 120 and that for β-carotene accumulation is 240. The isomers of β-carotene in D. salina cultured in different S were analysed by HPLC with an Alox-T alumina column. Six isomers of β-carotene were found in different proportions dependent on the S of the culture medium. All trans β-carotene increased from 37.2% to 47.8% when the S increased from 90 to 240, but both 9-cis and 15-cis β-carotene decreased from 32.4% and 22.3% to 26.1 % and 8.7% respectively. Decrease of S led to more total cis β-carotene accumulation.Because cis isomers of β-carotene are good anti-senscence substances and can also provent certain kinds of cancer. Proper lowering of the S is suggested for more cis β-carotene production in Dunaliella cultivation.