摘要
以苦丁茶树腋芽为外植体进行培养，诱导腋芽萌发，产生丛芽，同时，试管内外结合法诱导生根，并大田移栽．结果表明：ａ．初代培养以Ｂ５＋ＢＡ２ｍｇ／Ｌ＋ＩＢＡ０．５ｍｇ／Ｌ＋ＧＡ３０．５ｍｇ／Ｌ固体培养基诱导腋芽萌发较好；ｂ．Ｂ５＋ＢＡ０．５ｍｇ／Ｌ＋ＺＴ０．２ｍｇ／Ｌ＋ＧＡ３０．２ｍｇ／Ｌ固体培养基能诱导大量丛芽产生；ｃ．适当剪除叶片并去掉顶芽能促进腋芽萌发；ｄ．小苗在附加ＮＡＡ０．２ｍｇ／Ｌ，ＩＢＡ０．２ｍｇ／Ｌ，或ＮＡＡ０．１ｍｇ／Ｌ，ＩＢＡ０．２ｍｇ／Ｌ的１／２ＭＴ，１／２Ｂ５培养基上培养１０～１５ｄ，插入蛭石上发根，成苗快；ｅ．土壤保湿、避免强光照，是小苗成活的关键．
Germination of axillary bud and clump bud from cultured axillary buds, moth-ods of root formation and techniques of transplanting tube seedlings to field arereported inthis paper.a. Primary culture medium with B5+BA 2 mg/L+IBA 0.5mg/L+GA3 0.5 mg/L+agar 7 g/L is good for inducing axillary bud germination.b.A lot of clump buds areformed in B5medium with BA 0.5mg/L+ZT 0.2mg/L+GA3 0.2 mg/L.c. Resecting termi-nal bud and leaves suitablv can promote axillary bud germination.d.The root formation ofPlantlets which are cultured l0～15 days in1/2 MT or1/2 B5 medium with NAA 0.2 mg/L,IBA 0.2mg/Lor NAA 0.1mg/L,IBA 0.2mg/Lis optimum when they are inserted in ver-miculite.e. The key of plantlets transplanting is suitable soil moisture,avoiding strong sun-shineand the air temperature above 35℃.
关键词
冬青属
组织培养
培养基
苦丁茶
ilex,tissue cultures,culture media/axillary bud,Kudingcha
