摘要
本文用粉尘螨类为主的成螨制成螨体抗原片做间接荧光抗体试验,采用IgG荧光抗体,结果58例已确诊肺螨病患者的阳性率为94.8%,同时对其中20例肺螨病患者采用IgM荧光抗体作了IFA实验比较,仅3例出现了阳性反应。螨体显示荧光的主要部位在螯肢、足部和腹面。认为此法用于肺螨病的诊断具有较高的敏感性和特异性,可应用于临床肺螨病的辅助诊断。
Indirect fluorescent-antibody assay(IFA) by mixed mite antigen was established to determine the serum antibody of Pulmonary Acariasis.In IgG fluorescent-antibody group,the results showed that the positive rate in 58 cases was 94.8%,the parts which shoued fluorescence in mite were mainly chelicerace,podalic and ventral.In IgM fluorescent-antibody group,3 in 20cases with Pulmonary Acariasis showed fluorescence.The results suggested that the IFA by mixed mite antigen as a diagnostic method was sensitive and specific and had practical value of diagnosis for Pulmonary Acariasis.
出处
《济宁医学院学报》
1994年第3期5-6,共2页
Journal of Jining Medical University
关键词
肺螨病
螨体抗原片
诊断
Pulmonary Acariasis
IFA
Mite antigen