TDZ在棉花组织培养中的效应研究Ⅰ．TDZ单独使用对棉花组织培养的影响

Study of Effects on TDZ on Cotton Tissue CultureI.Effects of Single TDZ on Cotton Tissue Culture

ＴＤＺ是一种高效低毒棉花脱叶剂，它在棉花组织培养中表现出独特的生理作用。ＴＤＺ的添加促进了外植体的伸长和保绿，有利于外植体脱分化和胚性愈伤组织的诱导。但是ＴＤＺ不利于愈伤组织的保持和体细胞胚的发育，必须把在含ＴＤＺ培养基上诱导产生的愈伤组织转移到不含任何激素的培养基上，才能发育成成熟胚并再生出植株。ＴＤＺ的使用能诱导根的分化。不同外植体、不同基因型以及不同ＴＤＺ浓度之间在棉花组织培养中的表现均有差异。

The effects of a defoliate,thidiazuron(TDZ)(N- phenyl-N’-1,2,3-thiadiazol-5ylurea )on callus induction,subculture,differentiation,somatic embryogenesis and organogen-esis of vaious cotton varieties were studied.It was found that TDZ has unique physiologicalfuntion,Adding low dose of TDZ to basal n1edium can promote the growth and malntain greenof explants,and be beneficial to differentiation of explants and induction of embryogenic cal-lus,But it was disadvantageous to effectively maintain embryogenic callus and development ofsomatic embryos。It was absolutely necessary to transplant callus that has inducted from ex-plants on the medium adding various dose of TDZ to the medium without TDZ for somatic em- bryogenesis and obtaining mature embryos and plant recovery。Basic medium adding TDZ canpromote organogenesis also。 It has a various expression among explants,genotypes or various concentration TDZ in cotton tissue culture。