摘要
本文采用MTT比色法测定了LAK和TIL细胞培养上清IL-2活性及LAK细胞介导对体外培养的白血病细胞株的杀伤活性。结果表明LAK细胞和TIL细胞培养上清有较高的IL-2活性,可用于支持培养IL-2依赖株的生长;LAK细胞对体外培养的白血病细胞株有明显的杀伤作用。其杀伤效应随效靶比例增加而增高;同时亦证明:活的白血病细胞数与MTT甲赞形成产物呈直线相关性,提示通过MTT法测定OD值能较好地反映活细胞数目及细胞毒效应。
The activity of interleukine 2(IL-2) in culture supernatants of lymphokine-activated killer(LAK)cellsand tumor infiltrating lymphocytes (TIL)as well as cytotoxicity of LAK cells on cultured leukemic cells were detected by MTT colorimetry. The results showed that high activity of IL-2 in culture supernatants of LAK and TIL cells was found as well as it could be used for culture of IL-2 edpendent cell lines and clinic experiment. The significant cytotoxicity of LAK cells on leukemic cell lines could be found in vitro ,which was consistent with the ratio of effector cells to target cells. The number of living leukemic cells is consistently related with the concentration of formazan metabolite of MTT. It suggested that the numbers of living cells and cytotoxicity of LAK cells could be correctly estimated by the determina-tion of OD value.
出处
《免疫学杂志》
CAS
CSCD
北大核心
1994年第1期48-51,共4页
Immunological Journal
关键词
比色法
白细胞介素2
杀伤细胞
IL-2. LAK cells. MTT colorimetry. Leukemic cells. Cytotoxicity