摘要
溶脲脲原体(UU)是人泌尿生殖道的常见病原体,男性感染者又常伴有抗精子抗体,并可致不育。本实验证实了人精子与UU有共同抗原。① ELISA方法抗 UU抗体不仅可与UU抗原起反应,也可与人精子或精子膜发生反应;抗人精子抗体同样也可与UU抗原发生反应;②10%不连续SDS-PAGE可见人精子、精子膜提取物与UU有相同的蛋白电泳区带;③免疫印迹技术证明抗UUIgG对转移到硝基纤维薄膜上的人精子、精子膜和UU的电泳条带,在分子量 61KDa处发生特异性的交叉反应。
Ureaplasma urealyticum(UU)is a common pathogen in the human urogenital tract. The UU infected males often produced anti-sperm antibody and caused infertility. Our experiments demonstrated that there were presence of common antigen between human sperm and UU. The evidences were : (1) cross-immune reaction was present between anti-UU serum and human sperm or sperm membrane protein and vice versa$(2) Among UU, human sperm and sperm membrane protein,there were several similar protein electrophoresis bands in 10% discon-tinuons SDS-PAGE; (3) Among UU, human sperm and sperm membrane protein, there was similar 61 KD protein on nitrocellulose membrane in Western blotting by using of anti-UU IgG.
关键词
溶脲脲原体
共同抗原
精子
不育症
Ureaplasma urealyticum, Human sperm, Common antigen.