高效液相色谱法测定化妆品中对羟基苯甲酸酯类防腐剂

Determination of p-Hydroxybenzoic Acid Esters in Cosmetics by High Performance Liquid Chromatography(HPLC)

高效液相色谱法测定化妆品中对羟基苯甲酸酯类防腐剂王晓强，胡文炬，张军（北京进出口商品检验局北京１０００２２）１前言近年来，对羟基苯甲酸酯（甲、乙、丙、丁）被广泛地用作化妆品的防腐剂。许多国家对这类防腐剂在化妆品中的使用量有严格的限定，因此测定化妆品中...

new methodis described for the simultaneous determination of methyl , ethyl,n-propyl and n-butyl p-hydroxybenzoates(parabens)in cosmetics by HPLC. The method is simple, effective and accurate. After parabens are extracted with ethyl alcohol,the clear ethyl alcohol solution is passed through a neutral alununum oxide column to remove co-extracted lipids and pigments. Parabens are determined by reverse-phase HPLC at 254nm. The mobile phase is methanol-water witli a linear gradient from 60%to 80%of methanol in 20 min. Recoveries of four parabens were from 98.9%to 101.3%with coefficients of variation being less than 1.1%. This inethod is suitable for routine analysis of cosmetics.