鼠抗人IL－5单克隆抗体研制及初步应用探讨

IDENTIFICATION OF MONOCLONAL ANTIBODIES TO hIL-5 AND STUDY OF THE POSSIBILITY OF APPLICATION OF THE mAbs IN DETECTION OF HIL-5 CONCENTRATION

Ｔ细胞杂交瘤来源的人ＩＬ—５免疫小鼠。用鼠鼠杂交瘤技术获得两株分沁抗人ＩＬ—５特异性单抗（Ｓ１７和Ｓ６６）的杂交瘤，并对Ｓ１７单抗的特性进行了探讨。实验结果表明；两株单抗皆为ＩｇＧ１亚类；Ｓ１７单抗能显著抑制ＭＴＨｈ５Ｒ细胞的增殖，不能竞争性抑制ＮＣ１７单抗与人ＩＬ—５的结合。利用Ｓ１７单抗作为包被抗体建立的检测人ＩＬ—５ＥＬＩＳＡ法具有较高的敏感性，提示其在临床上检测病人血中ＩＬ—５浓度应用的可能性。

Two hybridoma (S17,S66) secreting monoclonal antibody against hIL-5 were established and the characteristic of S17 mAb was studied. Both S17 and S66 mAb are IgGl subtype. The competition inhibition assay demonstrated that S17 mAb cannot inhibit the combination of NC17 with hIL-5,which reveals that S17 combines with hIL-5 by a different epitope comparing with NC17 mAb. It's also proved that the mAb can inhibit the growth of hIL-5 dependent cell line,MTHh5R. The result of ELISA using S17 as a coating Ah showed that the mAb may have potential values for construction of hIL-5 detecting system in clinicwith both high sensitivity and specificity.