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小鼠全血中谷胱甘肽过氧化物酶活力的微量测定法 被引量:89

Direct Micro Determination of GlutatbionePeroxidase Activity in Mice Blood.
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摘要 报道了用分光光度计法直接测定小鼠全血中谷胱甘肽过氧化物酶GSH-PX活力的方法.取血样10μl,423nm为测定波长,三氯醋酸为蛋白沉淀剂,在pH6.5,3min的酶反应条件下,反应剩余的谷胱甘肽和其与DTNB试剂反应生成的颜色产物成线性相关.该法灵敏度高,重复性好,所需仪器简单。 direct micro determination of glutathioneperoxidase (GSH-PX) activity with spec-trophotometry was developed. Conditions ofthe assay were studied in detail. 10μl bloodsample was diluted with distilled water andtreated with 10% TCA to remove protein.There was a good linearity between DTNBproduct and concentration of GSH after 3 min-utes enzymatic reaction at the temperature of37℃ in PH 6.5 solution. The method hadhigher sensitivity, better reproducibility. Itmay became useful tool for analyzing GSH-PXin scientific research and clinical work.
出处 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 1994年第4期362-366,共5页 Progress In Biochemistry and Biophysics
关键词 谷胱甘肽 过氧化物酶 分光光度法 glutathione peroxidase ( GSH-PX), spectrophotometry, assay
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参考文献2

  • 1夏奕明,卫生研究,1987年,16卷,4期,29页
  • 2张嘉麟,中华医学检验杂志,1985年,8卷,199页

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