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四种溶脲脲原体对精子穿卵试验的作用比较 被引量:9

Comparing of Effects of Four Strains of Ureaplasma Urealyticum on Hamster Egg Penetration Assay by Human Spermatozoa
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摘要 分别用血清型4、9、10、12四种溶脲脲原体按20 ̄40颜色变化单位(CCU)/精子的浓度与正常生育男性精子一同孵育。结果发现,部分感染的精子尾部有绒毛状附着物;血清型4、12溶脲脲原体与精子共同孵育后,精子活率及活力明显下降,血清型9、10溶脲脲原体对精子活率及活力无明显影响。血清型4、9、10、12溶脲脲原体均使精子穿卵值(SPA)明显降低(分别为6.50%、10.45%、9.60%和6.40%),对照组为49.33%。提示溶脲脲原体不仅可能影响精子的活率与活力,而且可影响精卵相互作用乃至融合过程,溶脲脲原体的致病作用与其血清型有关。 Four strains of U. urealyticum, serotype 4, 9, 10, and 12, were incubated overnight withthe spermatozoa from normal fertile men respectively at the concentration of 20-40 CCU/spermatozoon. It was shown that fuzzy appearance was noticed at the tails of infected spermatozoa.Sperm viability and motility were declined dramatically after incubated with serovar 4 and 12,while serovar 9 and 10 being no significant effect on sperm viability and motility; significant decline of SPA was found after treated by U. urealyticum 4, 9, 10 and 12, the penetration rates being 6. 50%, 10. 45%, 9. 60% and 6. 45% respectively as compared with the normal value of 49.33%.The results inplied that U. urealyticum not only has deteriorating effect on sperm viability and motility, but also interfere with sperm-ovum interaction and/or fusion. A close relationship between U. urealyticum serovar and its pathogenicity was claimed in this study.
出处 《生殖与避孕》 CAS CSCD 北大核心 1994年第5期364-368,共5页 Reproduction and Contraception
基金 上海市科委自然科学基金
关键词 溶脲脲原体 血清型 精子穿卵试验 不育症 男性 U. urealyticum, Serovar, Spermatozoa penetration assay
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