摘要
用含400和800ppm17α-甲基睾酮的饲料投饲孵化后3天的异育银鲫仔鱼45天,再用无激素的饲料饲养1年,结果获得了一批性腺呈细线状、难辨雌雄的中性不育鱼。经随机解剖测定,中性不育鱼的比例分别是对照组的3.71和3.40倍;与对照组比较,试验组的平均体重分别增长16%和11%;平均性腺系数分别降低45.5%和24.5%;平均出肉率分别增长6.5%和5.0%。切片观察未发现中性鱼的性腺内有生殖细胞。试验表明,用400和800ppm的甲基睾酮都能导致雄激素阉割而产生中性不育鱼,以前者效果更好。经放射免疫测定,发现试验鱼血清和肌肉中的睾酮含量与对照组无显著差异。
The effect of feeding 17α-methyltestosterone (400 and 800ppm) over aperiod of 45 days, starting with 3-day-old fry, on growth and gonadal developme. ofallogynogenetic crucian carp was investigated. After the treatment, the tested fish wasreared with hormone-free diet for one year. The experimental results are as follows:Oraladministration of 17α-MT at 400 and 800 ppm resulted in a high percentage of sterile fish.The ratio of sterile fish was 3.71 times and 3.40 times as high as control group. Incomparison with the control group,the mean body weight in two experimental groupsincreased by 16% and 11 %,respectively.The mean gonadosomatic index decreased by45'5% and 24.5%, and the mean percentage of dress--out increased by 6.5% and 5.0%,respectively. Any germ cells were not been found in the thread--like gonad of sterile fish.The results suggest that oral administration of 17a--methyltestosterone at 400 ppm and 800 ppm to allogynogenetic crucian carp fry would result in androgenic castration and production of sterile fish, especially at the 400 ppm.The level of testosterone in serum and muscle of two experimental groups had no significant difference with control group, indicating the hormone--treated fish do not contain any residual hormones and they are quite safe for human consumption.
出处
《水产学报》
CAS
CSCD
北大核心
1994年第3期169-176,共8页
Journal of Fisheries of China
关键词
异育银鲫
中性不育鱼
性别控制
allogynogenetic crucian carp,sterile fish,17α-methyltestosterone,sex control,radioimmunoassay (RIA)