摘要
目的:建立山楂精降脂片中熊果酸含量的测定方法。方法:采用高效液相色谱法,色谱柱为Zorbax 80A Extend—C_(18)色谱柱(4.6 mm×250 mm,5μm),检测波长为220 nm,以甲醇-水-冰醋酸-三乙胺(83:17:0.04:0.01)为流动相,柱温35℃,流速为1.0 mL·min^(-1)。结果:熊果酸进样量在1.092-16.38μg范围内与峰面积呈良好的线性关系,r=1.000,平均回收率为99.2%。结论:本方法操作简便,精密度好,结果准确可靠。
Objective:To establish a method for determination of ursolic acid in Shanzhajing Jiangzhi tablets.Methods:To determine ursolic acid in Shanzhajing Jiangzhi tablets by HPLC.The liquid chromatography was carried out using a Zorbax 80A Extend -C_(18) column(4.6 mm×250mm,5μm)with detection wavelength at 220 run.The mobile phase:methanol-water-acetic acid-triethylamine (83:17:0.04:0.01).The flow rate was 1.0 mL·min^(-1).Results:The calibration curve was linear in the range of 1.092-16.38μg for ursolic acid (r=1.000).The average recovery(n=5)was 99.2%.Conclusion:The method is rapid,accurate and reliable.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2005年第2期237-238,共2页
Chinese Journal of Pharmaceutical Analysis