广东汉族人群HLA-ABDR基因频率分析

Analysis of HLA-A, B, DRB1 allele frequencies in Guangdong Han population

目的 检测广东汉族人群HLA -A ,B ,DRB1基因频率 ,分析该人群HLA等位基因多态性及其特点。方法 肾移植、骨髓移植供者 4 0 4例 ,抗凝血提取DNA ,半量全自动PCR -RSSO分型检测HLA -A ,B ,DRB1基因型。结果 在中分辨水平检出HLA -A等位基因 15个 ,HLA -B等位基因 31个 ,HLA -DRB1等位基因 13个。广东汉族人群HLA -A 11(0 .3342 ) ,A 0 2 (0 . 30 87) ,A 2 4 (0 . 2 2 16 ) ;HLA -B 6 0 (0 16 81) ,B 4 6 (0 . 16 81) ;HLA-DRB1 0 9(0. 175 5 ) , DRB1 15 (0 .14 90 ) ,DRB1 12 (0 . 1192 ) ,DRB1 14 (0 1192 ) ,DRB1 0 4 (0 .10 6 7)具有较高的基因频率分布 (>0. 10 )。Hardy -Weinberg平衡检验 :HLA -A(χ2 =6 0 .35 ,υ =5 4 ,P >0 1) ,B(χ2 =173. 6 7,υ =4 6 5 ,P >0 . 1) ,DRB1(χ2 =78. 4 6 ,υ =78,P >0 . 5 ) ,期望值和观察值吻合良好。结论 广东汉族群体HLA基因具有较为丰富的多态性 ,该人群HLA -A、B、DRB1等位基因处于遗传平衡状态 ,其基因频率分布具有地区性遗传特征。

Objective To analyze the polymorphism of HLA -A, B, DRB1 in Guangdong Han population and their HLA-A, B and DRB1 allele frequencies. Methods An auto semi-quantitative PCR-RSSO method was used to study the HLA-A, B and DRB1 genotypes of 404 samples of renal and bone marrow transplantation donors. Result 15 HLA-A, 31 B and 13 DRB1 alleles were obtained. HLA-A*11( 0.334 2 ),A*02( 0.308 7 ),A*24( 0.221 6 ),HLA-B*60( 0.168 1 ),B*46( 0.168 1 ),HLA-DRB1*09( 0.175 5 ),*DRB1*15( 0.149 0 ),DRB1*12( 0.119 2 ),DRB1*14( 0.119 2 ) and DRB1*04( 0.106 7 )showed higher allele frequency in Guangdong Han population. HLA-A, B, DRB1 in Guangdong Han population appeared to be in Hardy-Weinberg equilibrium. Conclusion HLA-A, B, DRB1 alleles in Guangdong Han population have rich polymorphisms. HLA-A,B,DRB1 allele is in genetic equilibrium in this population and their allele frequencies have the characteristics of area distribution.