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小鼠急性酒精性肝损伤模型的建立及应用 被引量:58

Establishment of mice acute liver injury model induced by alcohol and its application
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摘要 目的 建立小鼠急性酒精性肝损伤模型 ,并将该模型应用于保护酒精性肝损伤功能的保健食品的筛选与评价。方法 用体积分数为 5 0 %的乙醇造成肝损伤模型。实验分 4个实验组和 1个对照组 ,即 12ml/ (kg .bw)乙醇灌胃后禁食 6、8、10、12h处死动物 ;空白对照组给予蒸馏水 ,禁食 12h处死动物。取动物肝脏 ,冰冻切片、苏丹Ⅲ染色 ,进行病理组织学检查。依据《保健食品功能学评价程序和检验方法》(2 0 0 3年 )的方法和判断标准 ,用建立的模型测试某保健食品的护肝功能。结果 12h禁食组与空白对照组比较 ,前者呈现明显的肝脏损伤 ,病理改变以肝细胞脂肪变性为主 ,两者肝细胞脂肪变性评分分别为 2 2分、12分 ,平均秩次分别为 2 7 5和 9 79,差异有统计学意义 (P <0 0 1)。用该模型测试某护肝功能保健食品的结果显示 ,该受试样品各剂量组的脂肪变性评分均低于阳性对照组 (P <0 0 5 ) ;低、高剂量组能降低小鼠肝匀浆中过氧化脂质降解产物的含量 (P <0 0 5 ) ,各剂量组均能提高谷胱甘肽过氧化物酶的含量 (P <0 0 5或P <0 0 1)。结论 用体积分数为 5 0 %的乙醇一次灌胃染毒小鼠 ,并禁食 12h可成功造成肝损伤模型 ,并成功应用于护肝保健食品的筛选与评价研究。 Objective To establish the acute alcoholic liver injury model and apply in evaluation for health care foods. Methods Mice were randomized divided into five groups (4 dosage groups and one control group) and given 50%v alcohol. The four dosage groups were executed after fasting 6,8,10,12 hours respectively. Liver samples were observed pathologically with frost slice techniques and Sudan Ⅲ staining. The evaluation of liver function protection effects followed the national standard (2003). Results The pathological change of the test groups was fatty degeneration, fasting 12 hours group were remarkably different from the control group (P<0.01). The fatty degeneration grades and the mean ranks of these two groups were 22 and 12, and 27.5 and 9.79 respectively. The samples tested had certain liver protection function using this model. The fatty degeneration grade in every group was less than the positive control (P<0.05). MDA contents decreased significantly in low and high dosage groups (P<0.05). GSH-PX contents in all test groups were increased significantly (P<0.05 or P<0.01).Conclusion Mice acute liver injury model can be developed by given one dose of 50%v alcohol and fasted for 12 hours and can be applied in evaluating the liver protection of health care foods.
出处 《华南预防医学》 2005年第1期14-17,共4页 South China Journal of Preventive Medicine
基金 广东省医学科研基金资助项目 (B2 0 0 10 0 3 )
关键词 乙醇 肝疾病 酒精性 营养保健品 Ethanol Liver diseases,alcoholic Dietary supplements
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