一氧化氮血红蛋白等几种血红蛋白的拉曼光谱研究

Raman Spectral Study of Nitrosyhemoglobin and Several Other Hemoglobins

一氧化氮血红蛋白、高铁血红蛋白、氧合血红蛋白、去氧血红蛋白的拉曼光谱被比较研究。实验显示用 6 32 .8nm激发的拉曼光谱可检测氧合血红蛋白 (低自旋 )由R态到去氧血红蛋白 (高自旋 )T态的转变 ;同时一氧化氮血红蛋白与高铁血红蛋白的自旋态差异也可在拉曼光谱中清楚显现。6 32. 8nm激发的拉曼光谱可提供直接便利的方法鉴别一氧化氮血红蛋白及高铁血红蛋白这对相互关联的血红蛋白分子。

The Raman spectra of nitrosyhemoglobin(NOHb), methemoglobin(MetHb), oxygenated hemoglobin (oxyHb), and deoxygenated hemoglobin(deoxyHb) were comparatively studied. The results show that the Raman spectra with 632.8 nm excitation can be used to detect the R state (oxyHb) to T state (deoxyHb) transition. The difference of NOHb and MetHb in their electron spin state was clearly demonstrated in their Raman spectra with 632.8 nm excitation, suggesting that Raman spectra provide a new simple way to measure and distinguish NOHb and MetHb.