糖皮质激素对口腔扁平苔藓辅助性T细胞平衡的影响

Effects of glucocorticoids on T helper cells balance in oral lichen planus

目的 明确口腔扁平苔藓 (OLP)患者的Th1 /Th2免疫应答模式,探讨糖皮质激素对OLP患者辅助性T细胞平衡的影响。方法 密度梯度离心法分离OLP患者和对照组外周血单个核细胞,分别用植物血凝素(PHA)和地塞米松刺激OLP患者外周血单个核细胞,采用酶联免疫吸附法,检测培养上清液中干扰素γ(IFN γ)和白细胞介素 4(IL 4)的含量;应用半定量逆转录聚合酶链反应技术,检测培养细胞中IFN γ和IL 4mRNA的表达水平。结果 外周血单个核细胞经PHA诱导培养,OLP患者组IFN γ的水平低于健康对照组 (P<0 05),IL 4的水平高于对照组,但差异无统计学意义(P>0 05),IFN γ/IL 4的比值低于对照组 (P<0 05 )。地塞米松可抑制IFN γ和IL 4的水平(P<0 01),且对IFN γ的抑制作用较IL 4更为显著。结论 OLP患者存在Th1 /Th2的平衡失调,为Th2占优势的免疫应答,糖皮质激素对OLP的Th1 /Th2细胞因子均有抑制作用。

Objective To investigate the effects of dexamethasone on Th1/Th2 cytokines in oral lichen planus.Methods Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation from OLP patients and healthy controls. PBMC from patients with OLP were stimulated with phytohemagglutinin (PHA) and dexamethasone respectively for 72h. The concentrations of interferon γ(IFN γ) and interleukin 4 (IL 4) in culture supernatants were determined by ELISA. The mRNA levels for IFN γ and IL 4 in culture cells were evaluated using semi quantitative reverse transcription polymerase chain reaction.Results Compared with healthy controls, the levels of IFN γ in OLP patients were significantly lower( P <0 05). The levels of IL 4 were higher, but not statistically significant( P >0 05). The ratios of IFN γ/IL 4 were lower in patients with OLP( P <0 05). Dexamethasone significantly inhibited the levels of IFN γ and IL 4( P <0 01). Moreover, IFN γ was inhibited significantly more than IL 4. The levels of IFN γ and IL 4 mRNA expression in culture cells were consistent with protein production in supernatants.Conclusions Th2 immune response is predominant in OLP. Dexamethasone is an immunosuppressant inhibiting Th1/Th2 cytokines.