光气对小鼠MDA、GSH和SOD的影响

The Effect of Phosgene on MDA,GS Hand SOD in Mice

背景与目的 :研究小鼠光气染毒后不同时间对肺脏、血清和肝脏的氧化损伤。材料与方法 :40只雄性小鼠 ,随机分为4组。正常对照组小鼠以空气为对照 ,染毒组小鼠给予11.9mg/L剂量的光气 ,时间为5min,染毒后2、4、8h,测定各组小鼠肺脏、血清和肝脏的丙二醛(Malondialolehyde,MDA)和还原型谷胱甘肽(Glutathione,GSH)含量、总超氧化物歧化酶(TotalSuperoxideDismutase,T_SOD)活力。 结果 :随着光气染毒后时间的增加 ,小鼠肺脏、血清和肝脏的MDA含量升高 ;肺脏的T_SOD活力升高 ;肺脏和肝脏的GSH含量在染毒后2h降低。 结论 :光气染毒可引起小鼠肺脏、血清和肝脏的氧化损伤。

BACKGROUND&AIM: To study oxidative injury of lung serum and liver in different time in mice after exposed to phosgene. MATERIAL AND METHODS: 40mice were randomly divided into4groups with10mice in each.The mice in negative group were exposed to room air and the mice in positive group were exposed to11.9mg/L phosgene for5minutes,respectively.After2hours',4hours',8hours'exposure to phosgene,all mice were killed.The content of malondialdehyde(MDA)and reductive glutathione(GSH),activities of the total superoxide dismutase(T-SOD)in lung,serum and liver were determined. RESULTS: With the time prolonged after the mice were poisoned,MDA content in lung,serum and liver significantly increased(P<0.05);T-SOD activity significantly increased in lung(P<0.05);GSH content significantly decreased in mice lung and liver in negative group compared with that of negative group2hours after phosgene exposed(P<0.05). CONCLUSION: Phosgene can induce pulmonary edema and oxidative injury of lung serum and liver in mice.