摘要
目的 :探讨创伤应激状态下小鼠细胞免疫功能变化及黄芪多糖 (APS)对其影响。方法 :采用小鼠截肢应激模型 ,将 5 0只Balb/c小鼠随机分为 5组 :正常对照组 (A) ;应激对照组(B) ;应激 +APS高 (C)、中 (D)、低 (E)剂量组 ,每组 10只。免疫组织化学技术检测小鼠胸腺、脾脏组织中CD4 、CD8、c -fos蛋白表达 ,计算机图像分析系统定量。结果 :与正常对照组 (A)比较 ,创伤后 72小时应激对照组 (B)小鼠CD4 抗原分布、CD4 /CD8比值显著减少 (P<0 0 1) ,c -fos抗原分布明显多于正常 (P <0 0 1) ;与创伤应激对照组 (B)比较 ,应激 +APS高 (C)、中 (D)、低 (E)剂量组小鼠CD4抗原水平、CD4 /CD8比值升高 (P <0 0 1)、c-fos抗原水平降低 (P <0 0 1) ;与正常对照组 (A)比较 ,应激 +APS高剂量组 (C)小鼠CD4、CD8、c -fos抗原分布无显著差异 (P >0 0 5 )。结论 :创伤后小鼠细胞免疫功能紊乱 ;黄芪多糖可有效恢复其细胞免疫功能。
To investigate the effects of traumatic stress and Astragalus Polysaccharide on cell immunity of mice amputee model. 50 BALB/C mice were divided into 5 groups randomly, normal control group (group A), traumatic stress group (group B), traumatic stress+high dose APS group (group C), traumatic stress+middle dose APS group (group D), traumatic stress+low dose APS group (group E). CD 4,CD 8 and expression of c-fos protein were detected by immunohistochemisty method. Expression of NF-κB mRNA and IL-10 mRNA were detected by hybridization in situs.Their results were quantitated by HPIAS-1000 system. Compared with that of group A, the expression level of NF-κB mRNA and IL-10mRNA in thymus and spleen tissues of group B mice after injury 72 hours were elevated significantly(P<0.01),CD 4 and CD 4/CD 8 ratio were reduced(P< 0.01), the distribution of c-fos antigen more than that of group A.Compared with group B , the level of expression of NF-κB mRNA and IL-10 mRNA in thymus and spleen tissues were not only depressed by APS and the level of CD4 antigen, CD 4/CD 8 ratio were elevated in groups C,D,E,but thelevel of c-fos antigen were reduced(P< 0.01). Between the group B and the groupC,the expression of NF-κB mRNA and IL-10mRNA, CD 4 and CD 4/CD 8 ratio, the distribution of c-fos antigen had no significant difference (P>0.05). The cell immuni ty function of mice were disordered after their trauma,but APS may play a role in restoring their cell immunity.
出处
《中国体视学与图像分析》
2004年第2期91-94,共4页
Chinese Journal of Stereology and Image Analysis
