草棉花总黄酮对大鼠血小板聚集及脑血栓形成的影响

The effects of the active principle of Flos Gossypium Flavonoid(FGF) on platelet aggregation and cerebral thrombosis in rats

目的: 研究草棉花总黄酮对血小板聚集功能及对大鼠脑血栓形成的影响。方法: 采用比浊法测定血小板聚集功能,放免法测定血浆中TXB2、6 keto PGF1a的含量,经颈内动脉注射复合血栓诱导剂(1 ml/kg)诱发大鼠右侧大脑半球内血栓形成,观察总黄酮对大鼠脑电图、脑重量、脑内伊文思蓝含量的影响和脑病理切片。结果:总黄酮(50、200 mg/kg×7 d)对3种血小板聚集诱导剂分别引起的大鼠体外血小板聚集有抑制作用(P<0.01),其中剂量为200 mg/kg的总黄酮对ADP诱导的血小板聚集的抑制率为75%,对胶原诱导的血小板聚集的抑制率为73%,对凝血酶诱导的血小板聚集的抑制率为67%。总黄酮(50、200 mg/kg×7 d)可降低大鼠血浆中TXB2 的水平,增加大鼠血浆中6 keto PGF1a水平(P<0.01),并能对抗大鼠大脑血栓形成(P<0.01)。结论: 总黄酮可能通过影响血浆中TXA2 和PGI2 的含量而抑制血小板聚集从而发挥抗血栓作用。

Objective: In this article we studied the influence of the active principle of Flos Gossypium Flavonoid (FGF) on platelet aggregation and thrombosis in rats. Methods: We assessed platelet functions by turbidimetry and TXB\-2 and 6-keto-PGF 1α were measured through radio-immunological methods. Cerebral thrombosis in the right hemisphere in rats was induced by the injection of composite thrombosis inducers into internal carotid artery and the effects of FGF on electroencephalogram, brain weight, Evans Blue content and brain pathology were observed. Results: The results showed that FGF (50、 200 mg/kg×7 days) can inhibit platelet aggregations which are produced by inducers of platelet aggregation in vitro. The inhibition rats of FGF(200 mg/kg) on the platelet aggregation caused by ADP, collagen and thrombin were 75%, 73% and 67%, respectively. FGF(50、 200 mg/kg×7 days) can reduce the plasma TXB\-2 content in rats and increase its 6-keto-PGF 1α. We found that FGF (50、200 mg/kg×7 days) antagonizes cerebral thrombosis in rats. Conclusion: FGF may exert an anti-thrombosis action by inhibiting platelet aggregation through influencing plasma TXB\-2 and PGI\-2 contents.