彩绒革盖菌产漆酶及其对染料脱色的研究

STUDIES ON LACCASE PRODUCTION BY CORIOLUS VERSICOLOR AND ENZYMATIC DECOLORATION OF DYE

采用彩绒革盖菌 (Coriolusversicolor)在液体深层发酵条件下产漆酶,优化后的最适工艺条件为:以 1. 1g/L蔗糖与 1. 1g/L葡萄糖作为复合碳源,氮源为 1. 88g/L豆饼粉,初始pH值 4. 5,培养温度 30℃,漆酶活力可达 18. 2U/mL。漆酶的最适反应温度为 60℃,在 55℃以下酶活力均保持稳定。综合考虑漆酶作用的最适温度与热稳定性,确定该酶的适宜应用温度为 55℃。利用粗酶液对染料酸性橙进行降解脱色试验,发现氧化还原介质可明显提高漆酶的催化能力,作用 5h最高脱色率可达 96. 5%,显示了漆酶在环境治理方面良好的应用前景。

Laccase production by a white-rot fungus Coriolus versicolor was studied under submerged fermentation (condition).The optimal fermentation parameters were as follows:carbon source 1.1 g/L sucrose combined with 1.1 g/L glucose,nitrogen source 1.88 g/L bean cake powder,culture temperature 30 ℃ and initial pH value 4.5.The activity of laccase reached 18.2 U/mL.The optimum reaction temperature of laccase was 60 ℃,and laccase was stable when the environmental temperature was below 55 ℃.Considering the thermal stability of laccase,the suitable temperature for the enzymatic application was 55 ℃.The laccase produced by C.versicolor could hydrolyze and decolorize aromatic dye.During the decoloration of acid orange dye with laccase,the addition of redox mediators could obviously increase the catalytic ability of the enzyme.The decoloration rate of acid orange dye could reach 96.5% after 5 h,which showed a good application potential in environment protection.