大鼠急性全脑缺血致脑-肺综合征模型的建立及评价

The Creation and the Evaluation of th e Cerebro -Pulmonary Syndrome Model Following Acute Ommi-Cerebral Isch emia in Rats

目的建立并评价大鼠急性全脑缺血致脑-肺综合征模型,为探讨急性缺血性脑血管疾病并发脑-肺综合征的发病机制和临床防治奠定基础。方法随机将48只大鼠分为正常组、假手术组及模型组四个亚组(即再灌注后1h,3h,6h,12h),采用四动脉阻断法制作全脑缺血再灌注致脑-肺综合征模型,结合呼吸频率、血气分析、白细胞计数、肺组织含水量、热休克蛋白70(HSP70)表达及肺组织病理观察,评估该动物模型。结果该模型动物不仅存在明确的脑缺血,而且短时间内即出现一系列炎性反应,出现严重的肺功能损伤和明确的肺组织病理改变。结论大鼠急性缺血再灌注可成功地模拟急性脑缺血后并发脑-肺综合征的病理过程。

Objective:To create and evaluate the cerebro -p ulmonary syndrome model following a -cute omni -cerebral ischemia in rats so as to lay a foundation for approach ing the pathogenesis and the prevention and treatment of cerebro -pulmonary syn drome following acute ischemic cerebrovascular sie ase.Methods:48rats were randomly divided into th e normal group,the pseudo -operation group,and the model group which consisted of 4subsections (1,3,6,12h after reperfusion).The method of blocking 4arteries wa s ap-plied to make cerebro -pulmonary syn drome model following acute omni -ce rebral is-chemia reperfusion,and the model wa s evaluated by respiratory rate,blo od gas analysis,numeration of leukocyt,weight of wa ter in the lung tissues,expression o f HSP 70in the brain tissues and pathomorphology o f the lung tissues.Results:It appeared that the ani-mal model had not only definite cereb ral ischemia but also severe pulmonary function lesion and definite change of pathom orphology of the lung tissues result ing from a series of inflammatory reactions in a short time.Conclusion:Acute cerebral ischemia reperfusio n in rats can successfully mimic the pathologic coures of cerebro -pulmonary syndrome model following acute cerebral isch emia.