6种白腐菌腐朽后的山杨木材和木质素官能团变化的红外光谱分析

FTIR Analysis on Function Groups of David Poplar Wood and Lignin Degraded by 6 Species of Wood White-Rot Fungi

选择火木层孔菌及 5种木材降解能力较强的阔叶树上的白腐菌 :粗毛盖菌、偏肿拟栓菌、三色革裥菌、冬拟多孔菌和血红密孔菌 ,采用国内外红外光谱分析的标准方法 ,用傅里叶红外光谱仪测定未腐朽材木粉和受 6种白腐菌腐朽 12 0d后的腐朽材木粉试样的红外光谱图。刮取未腐朽的山杨木材样品和受 6种白腐菌腐朽 12 0d时的山杨木材样品表层少许 ,在干燥条件下 ,分别放入KBr中 ,磨细 ,压片 ,然后在FTIR光谱仪上进行测定 ,得到经 6种白腐菌降解 12 0d后的木材木粉和未腐朽材木粉其木材和木质素官能团谱峰位置和谱峰相对吸收强度的振动变化状况 ,进而分析腐朽后的山杨木材和木质素官能团的变化情况 ,以作为木材白腐菌对山杨材生物降解机制的进一步研究。结果表明 ,受 6种白腐菌腐朽后的山杨木材和木质素官能团都受到一定程度的降解 ,但各自的变化情况有所不同。对木质素的降解主要是存在于侧链上 ,虽然苯环骨架变化不明显 ,但木质素苯环间的羰基、CH2 结构、紫丁香基和愈疮木基等侧链已部分被降解。从各吸收峰相对吸收强度的变化大小来看 ,血红密孔菌、冬拟多孔菌。

In order to offer an applicating basic theory study to biopulping with David Poplar wood, and decrease environment pollution in paper industry and make full use of lignin resources, this study selected Phellinus igniarius and 5 wood-rotting fungi with higher wood degrading ability living on broad-leaf trees, i.e. Funalia gallica, Pseudotrametes gibbosa, Lenzites tricolor, Polyporellus brumalis and Pycnoporus sanguineus, FTIR spectrometer was used to measure infrared spectrograms of primitive wood sample and wood samples degraded by 6 species of fungi on 120 d respectively, a little powders shaved from the very surface of primitive wood sample and wood samples degraded by 6 species of fungi on 120 d were put into KBr powder and milled under dryness condition, then pressed to slices and put FTIR to measure infrared spectrograms, libration changes of peak location and relative absorption intensity of primitive wood sample and wood samples degraded by 6 species of fungi on 120 d were gained, then the changes of function groups of wood and lignin after rotted were analysed in order to get more study on wood biodegradation. Results showed the function groups of wood and lignin were all degraded in different degree. Although aromatic rings were not changed very much, side chains of lignin such as carbonyl groups, CH2, syringic units and guaiacyl units were partly degraded. The extents of lignin degraded by 6 species of wood white-rot fungi were different in the view of peak absorption intensity change, P. sanguineus, P. brumalis, L. tricolor and P. gibbosa had higher ability than F. gallica and P. igniarius in lignin degrading.