应用原生质体融合技术改善双歧杆菌的耐氧性

Study on Improving Oxygen-resistance of Bifidobacterium by Using Protoplast Fusion Technique

利用原生质体融合技术将专性厌氧的短双歧杆菌和典型上面发酵的啤酒酵母进行原生质体细胞融合,通过研究两亲本原生质体的形成与再生,并进行跨界融合,成功初筛出兼具两亲本生物学性状、较稳定的融合子BSF1和BSF2,改善了双歧杆菌的耐氧性。

The protoplast fusion between Bifidobacterium breve and Saccharomyces cerevisiae was studied by using protoplast fusion technique, a pair of hybrid fusants BSF1 and BSF2, comparatively stable and having biological properties of both Bifidobacterium breve and Saccharomyces cerevisiae, were obtained successfully after forming, regeneration and fusion of protoplast of two parents was finished .The oxygen-resistance of Bifidobacterium was improved.