呼吸型鸡传染性支气管炎病毒的抗原定位与动态分布

Antigen localization and dynamic distribution of respiratory infectious Bronchitis virus

应用间接免疫荧光抗体(IF)和RT-PCR法对人工感染IBV-M41株的SPF鸡不同脏器中的病毒进行了跟踪检测.结果在感染后24 h气管中最早出现特异性荧光,感染后第3 d肺脏和肾脏出现特异性荧光,感染后第5d肝脏、脾脏、法氏囊、直肠等脏器也不同程度的出现特异性荧光.特异性荧光在气管中最强,持续时间也最长,可达14d或更长;在肺脏和肾脏可持续3～5d;而在其他器官持续1～3d.试验结果表明气管、肺脏、肾脏都是M41株病毒增殖的场所,但随感染时间的延长,病毒最终定位于主要的靶器官气管.IFA和RT-PCR方法均可以对IBV抗原定位,相对来说IFA法更直观,而RT-PCR更灵敏.

IFA and RT-PCR were used to detect difference organs of SPF chickens infected IBV-M41 strain. The results showed that the first bright specific fluorescence (BSF) was observed in chicken trachea epithelial cells with specific staining 24hrs after artificial IBV-infection. The BSF was observed in chicken lungs' and kidneys' cells 3days after artificial IBV-infection. There were different degrees of BSF in cells of liver, spleen, bursa and rectum 5days after artificial IBV-infection. The BSF was most noticeable in trachea and its lasting time could last for 14days or longer. The BSF could last for 3-5days in lung and kidney, and could last for 1-3days in other organs. It was showed that trachea, lungs and kidneys were the place of virus copying, with the time of IBV-infection, the virus were located in the target organ-trachea. IFA and RT-PCR were both used for antigen localization, IFA was more direct, and RT-PCR was more sensitive and specific.