赤芍总苷对沙土鼠全脑缺血再灌注损伤的保护作用

Protective effects of total paeony glycoside against global cerebral ischemia-reperfusion injury in gerbils

目的观察赤芍总苷(TPG)对全脑缺血再灌注的保护作用。方法采用结扎双侧颈总动脉12min再灌注24h建立沙土鼠全脑缺血再灌注模型,观察于造模24h后TPG对沙土鼠脑组织含水量、SOD与MDA及病理组织学的影响。结果同模型组比较,TPG200、400mg/kg·b.w.组脑组织含水量明显低于模型组;模型组脑组织SOD活性明显降低,MDA含量明显提高,各给药组与模型对照组比较SOD明显升高,MDA含量显著下降;病理检查表明给药组的病理损伤较模型组轻。结论赤芍总苷对全脑缺血再灌注损伤有明显的保护作用。

Objective To study the protective effects of the total paeony glycoside (TPG) against global cerebral ischemia- reperfusion injury in gerbils. Methods Gerbils models of global cerebral ischemia-reperfusion injury were prepared by bilateral common carotid artery ligation for 12 min followed by 24-hour reperfusion. The effects of TGP on brain edema index, superoxide dismatase (SOD) activity and malonaldehyde (MDA) concentration of the cerebral tissue homogenate and pathology of the brain were examined 24 h after model establishment. Results Compared with the model group, TPG at the doses of 200 and 400 mg/kg could significantly relieve brain edema, enhance SOD activity and lower MDA concentration in the gerbils. Pathological examination showed that the gerbils with TPG treatment had milder injury of the cells in the hippocampal CA1 region. Conclusions TPG has obvious protective effects against global cerebral ischemia-reperfusion injury.