烫伤、内毒素对豚鼠胃肠动力影响的实验研究

EXPERIMENTAL STUDY OF THE EFFECT OF BURN AND LPS ON GUINEA PIG'S GASTROINTESTINAL MOTILITY

目的:探讨烫伤后胃肠动力障碍发生机制。方法:豚鼠30只,随机分为烫伤、内毒素及对照组,在烫伤及内毒素腹腔注射1小时后测定豚鼠胃肠推进距离、肠道组织降钙素基因相关肽(CGRP)、Na+ -K+ 、Mg2 + 、Ca2 + 、及Ca2 + -Mg2 + -ATP酶含量。结果:①烫伤及内毒素组肠道推进功能受到抑制,烫伤组抑制更明显。②烫伤及内毒素组CGRP含量较对照组增多,烫伤组增高幅度更明显。③烫伤及内毒素组豚鼠肠道组织中各ATP酶的含量较对照组明显减少,两组ATP酶减少程度无明显差异。结论:①烫伤及内毒素抑制豚鼠的肠道推进功能,烫伤比内毒素组抑制更明显。②烫伤及内毒素组CGRP含量较对照组明显增多,烫伤组CGRP增高幅度更明显。③烫伤及内毒素组各ATP酶的含量较对照组减少,烫伤组、内毒素组各ATP酶减少程度无明显差异。

Objective: To study the mecha n ism of the dysfunction of gastrointestinal motility after burn. Meth ods: 30 Guinea pigs were randomly divided into 3 groups: burn group ,Lipopolysaccharide(LPS) treated group and normal control group. Animals were k illed 1 hour after being burned and LPS injected, then the quantity of the CGRP, Na+-K+-ATP enzyme, Mg 2+-ATP enzyme, Ca 2+-ATP enzyme, and Ca 2+- Mg 2+-ATP enzyme in gastrointestinal tissue were measured. Results: ①The propelling function of gastrointestinal tube in bur n group and Lipopolysaccharide(LPS) treated group are restricted, it is more obv ious in burn group. ②The quantity of the CGRP in burn group and LPS treated gro up is more abundant than in control group,the degree of increasing in burn group is more obvious. ③The quantity of Na+-K+-ATP enzyme, Mg 2+-ATP enzyme , Ca 2+-ATP enzyme, and Ca 2+-Mg 2+-ATP enzyme in gastrointestina l tissue decrease in burn group and LPS treated group, and there was no obvious difference between burn group and LPS treated group. Conclusions: The in crease of CGRP and the decrease of ATP enzymes in burn group an d LPStreated group might play a role in the weakening of gastrointestinal tract motility.