高活性干酵母对中华鳖非特异性免疫功能和抗病力的影响

Effect of Activity Yeast Cell on Non-specific Immune Function and Disease Resistance in Juvenile Soft-shelled Turtles (Pelodiscus sinensis) by Oral Administration

报道了高活性干酵母对中华鳖(Pelodiscus sinensis)幼鳖非特异性免疫功能和抗病力影响的研究结果。试验共设6个组,在各组饵料中高活性干酵母的添加量依次为每千克体重0 mg(对照组)、600.0 mg(试验I组)、800.0 mg(试验Ⅱ组)、1 000.0 mg(试验Ⅲ组)、1 200.0 mg(试验Ⅳ组)和1 400.0 mg(试验Ⅴ组)。投喂30 d后,从每组中各取10只中华鳖幼鳖采血,测定了中华鳖幼鳖血液中白细胞的吞噬活性、血清中溶菌酶和补体活性;另从每组分别取30只中华鳖幼鳖用注射嗜水气单胞菌(Aeromonas hydrophila)的方法行人工感染,检测其抗病力。研究结果表明,各试验组中华鳖幼鳖血液中白细胞的吞噬活性均显著高于对照组。第Ⅰ和第Ⅱ组中华鳖幼鳖血清中溶菌酶活性与对照组之间没有显著性差异,而试验Ⅲ、Ⅳ组和Ⅴ组中华鳖幼鳖血清中溶菌酶活性均显著高于对照组。第Ⅱ、Ⅲ、Ⅳ和第Ⅴ组中华鳖幼鳖血清中补体活性与对照组之间存在显著差异,而第Ⅰ组与对照组之间无显著性差异。投喂高活性干酵母能增强中华鳖幼鳖对人工感染A. hydrophila活菌的抵抗力。研究结果证明在中华鳖幼鳖饵料中添加高活性干酵母的量以每千克体重1 200.0 mg左右为宜。

The effect of the dietary activity yeast cell on non-specific immune function and disease resistance in juvenile soft-shelled turtle (Pelodiscus sinensis) was studied. The turtles were divided into 6 groups and were fed with feed supplemented with different ratio of activity yeast cell,which was 0 mg/kg(control group), 600.0 mg/kg(group I), 800.0 mg/kg(group Ⅱ), 1000.0 mg/kg(group Ⅲ), 1200.0 mg/kg(group Ⅳ)and 1400.0 mg/kg(group Ⅴ) respectively. 30 days later, the turtles were blooded, and the phagocytic activity of the leucocytes, serum lysozyme activity, complement activity and disease resistance were monitored. The results showed that the phagocytic activity of the leucocytes of the expermental groups were significantly higher than that of the control group (t-test, P<0.05).The lysozyme activity of the serum in group Ⅲ, Ⅳ and Ⅴ were significantly higher than the control group (t-test, P<0.05). However, there were no significant differences among group Ⅰ, Ⅱ and the control group (t-test, P>0.05). The highest complement activity of the serum in group Ⅱ, Ⅲ, Ⅳ and Ⅴ were significantly higher than the control group (t-test, P<0.05), while there were no significant differences between group Ⅰ and control group (t-test, P>0.05). The results demonstrate that the best supplementation ratio of the activity yeast cell in the soft-shelled turtle is about 1200.0 mg/kg. B.W.