川芎嗪对培养人脐静脉内皮细胞基因表达谱的影响

Ligustrazine-induced Alterations in Gene Expression in Human Umbilical Vein Endothelial Cells

目的:研究川芎嗪对培养人脐静脉内皮细胞基因表达谱的影响,探讨川芎嗪保护内皮细胞的分子机制。方法:制备包含5 0 0个心血管疾病相关基因的寡核苷酸芯片;运用制备的心血管寡核苷酸芯片研究川芎嗪对培养人脐静脉内皮细胞基因表达谱的影响。结果:川芎嗪(40微克 毫升)作用培养内皮细胞2 4小时后,寡核苷酸芯片分析筛选出川芎嗪对内皮细胞的影响基因2 5个,其中上调基因7个,下调基因18个,包括一些与免疫、血管舒缩、细胞粘附、凝血、抗氧化、细胞生长、信号转导及物质代谢的相关基因。结论:川芎嗪在基因水平通过多环节调节内皮功能。

To investigate the effect of Ligustrazine on the spectrum of gene expression in the cultrured human umbilical vein endothelial cells(HUVECs),and further explore the potential molecule mechanism of endothelial protection by Ligustrazine on HUVECs.Method:A home-made oligonucleotide microarray containing 500 cardiovascular disease-related genes was constructed.The alteration of the spectrum of gene expression induced by Ligustrazine in HUVECs was detected by oligonucleotide microarray analysis.Results:7 up-regulated and 18 down-regulated genes were obtained after Ligustrazine (40ug/mL) had acted on HUVECs for 24hours.Ligustrazine affected the expression levels of genes involved in immunization,vasomotion,cell adherence,coagulation,antioxidation,cell growth,signal transduction and substance metabolism in HUVECs.Conclusions:Ligustrazine regulated sets of genes in endothelial cells and protected the cells.Microarray analysis provided us valuable insights into the protective mechanism by Ligustrazine.