壬基酚对性成熟期F_1子代雄性SD大鼠生殖发育的影响

Adverse Effects of Nonylphenol on the Reproductive Development of F_1 Male SD Rats in Sexual Maturation Period

目的 研究壬基酚(NP)对性成熟期F1 子代雄性SD大鼠生殖发育的影响。方法 设NP5 0 mg/ kg,10 0 m g/ kg,2 0 0 m g/ kg三个剂量组和一个空白对照组,对母鼠从妊娠第7d至断乳期灌胃染毒,产后5 5 d处死雄性子代,检测血清睾酮,取附睾作精子计数和活度测定,取睾丸作病理组织学观察和细胞增殖核抗原(PCNA)、雌激素受体(ER)及芳香化酶(Arom)的免疫组化分析。结果 与对照组相比,2 0 0 m g/ kg NP组血清睾酮浓度、精子计数、精子活度显著降低(P<0 .0 5 ) ;睾丸组织病理学检查显示,发育异常的生精小管数增多,支持细胞数量减少,生精功能受损;2 0 0 mg/ kg NP组PCNA的表达显著低于对照组(P<0 .0 5 ) ;10 0 mg/ kg和2 0 0 mg/ kg NP组Arom的表达显著降低(P<0 .0 5 ) ;各染毒组ER的表达与对照组相比无显著性差异。结论 2 0 0 mg/ kg NP能明显损伤性成熟期F1 子代雄性SD大鼠的生殖发育功能。

Objective To investigate the adverse effects of perinatal exposure to nonylphenol(NP) on the reproductive development of F_1 male SD rats in sexual maturation period. Methods The pregnant rats were randomly divided into control, 50 mg/kg, 100 mg/kg, and 200 mg/kg NP groups respectively. NP was administered to dams by gavage from gestation day 7 to weaning period. Rat pups were sacrificed at postnatal day 55. The concentration of serum testosterone was measured by radioimmunoassay. The epididymis was subjected to the determination of sperm count and motility while the testis was submitted to histopathological and immunohistochemical analyses. Results Compared with control, the concentration of serum testosterone, sperm count and motility in the 200 mg/kg NP dose groups significantly decreased (P<0.05), and the histopathological examination revealed that NP-treated groups had higher rates of maldeveloped siminiferous tubules, smaller amount of Sertoli cells and weaker spermatogenesis. The expression of proliferative cell nuclear antigen(PCNA) significantly decreased in the 200 mg/kg NP dose groups (P<0.05). The expression of Arom in 100 mg/kg and 200 mg/kg NP dose groups was lower than that in control. There was no significant difference in ER expression between the control and NP-treated groups. Conclusion These findings indicated that 200 mg/kg Nonylphenol apparently damaged the reproductive development of F_1 male SD rats in the sexual maturation period.