TGF-β1和MMP-2在胆囊癌中表达及临床意义

Expressions and clinical significance of TGF-β1, MMP-2 in gallbladder carcinoma

目的:检测转化生长因子β1、基质金属蛋白酶-2在胆囊癌中的表达情况,研究它们在原发性胆囊癌发生、发展过程中的表达特点及相互关系.方法:应用免疫组化SP法检测36例原发性胆囊癌中TGF-β1、MMP-2的表达,并以同期20例慢性胆囊炎作对照.结果:1)TGF β1、MMP2基因蛋白阳性表达率分别为63.89%(23/36)、52.78%(19/36),显著高于其相应良性对照组10.00%(2/20)和5.00%(1/20).2)TGFβ1在有淋巴结或远处转移、NevinⅣ～V期患者中阳性表达率(79.17%)明显增高于无转移、NevinⅠ～Ⅲ期的患者(33.33%).MMP-2阳性表达主要集中在低分化、有淋巴结或远处转移的患者中,P＜0.05.3)MMP2与TGF-β1两者呈正相关,P＜0.05.4)TGF-β1阳性患者生存率低于TGF-β1阴性患者,P＜0.05,MMP-2阳性患者生存率低于MMP-2阴性患者,P＜0.05.结论:1)TGF-β1、MMP-2阳性表达主要集中在低分化、有淋巴结或远处转移的患者中,P＜0.05.2)联合检测TGF-β1、MMP 2在原发性胆囊癌中表达有助于反映原发性胆囊癌生物学特性,为预后判断提供参考指标,并为干预性基因治疗提供实验依据.

OBJECTIVE: To investigate the expressions of TGF-β1 and MMP-2 in gallbladder carcinoma and their relation to carcinoma development.METHODS:The expressions of TGF-β1 and MMP-2 in gallbladder carcinoma were detected by SP immunohistochemical staining, 20 cases of chronic cholecystitis were collected as contrast.RESULTS: 1)The positive rates of TGF-β1(63.89%) and MMP-2(52.78%) in gallbladder carcinoma increased significantly,P<0.05. 2) The positive rate of TGF-β1 was higher in metastasis or Nevin Ⅳ-Ⅴ stage group of gallbladder carcinoma than that was in non-metastasis or NevinⅠ-Ⅲ stage group,P<0.05. In metastasis or low differentiation there was also higher expression of MMP-2,P<0.05. 3) There was a statistically significant positive correlation between the expressions of MMP-2 and TGF-β1, P<0.05. 4) There was a significant difference in survival time between patients with TGF-β1(+) and ones with TGF-β1(-). The difference was also found between patients with MMP-2(+) and ones with MMP-2(-). There was a statistically correlations between the first group and the second group.CONSLUSIONS: 1)The positive rate of TGF-β1 is higher in metastasis or Nevin Ⅳ-Ⅴ stage group of gallbladder carcinoma than that is in non-metastasis or Nevin Ⅰ-Ⅲ stage group,P<0.05 .In metastasis or low differentiation there is also higher expression of MMP-2, P<0.05. 2) The detection of TGF-β1 and MMP-2 may be useful in assessing the development of cancer and judging the prognosis, and eventually render a possible target for novel therapeutic strategies.