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3种提取胶带粘面汗潜指印中DNA的方法比较 被引量:11

Compare with the three methods of DNA extraction of sweat latent fingerprint on the adhesive side of tapes
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摘要 目的比较胶带粘面汗潜指印中DNA提取的方法。方法分别采用硅珠法、QIAMicrokit法、硅珠-QIAMicrokit法提取胶带粘面的汗潜指印中DNA,STR复合扩增,荧光电泳检测。结果用QIAMicrokit法、硅珠-QIAMicrokit法提取胶带粘面汗潜指印中DNA,检测成功率分别为21%和36%。硅珠法检测未获成功。结论硅珠-QIAMicrokit法提取胶带粘面汗潜指印中的DNA比QIAMicrokit法,检验时间更短,检测成功率更高。 Objective To comparatively analyze the DNA extracted from sweat latent fingerprints on the adhesive side of tapes by three kinds of methods. Methods DNA was extracted from sweat latent fingerprint on the adhesive side of tapes using silicon bead test,QIA micro Kit and combined silicon bead-QIA micro Kit. STR loci were detected by multiplex PCR procedures. The PCR product was electrophoresed on an ABI 3100 Genetic Analyzer. Results 36%of the sweat latent fingerprints on the adhesive side of tapes was successfully genotyped using combined silicon bead-QIA micro Kit, and 21% using QIA micro Kit. Conclusion DNA genotyping of the sweat latent fingerprints on the adhesive side of tapes reveals higher possibility using combined silicon bead-QIA micro Kit than using QIA micro Kit and is less time-consuming.
出处 《中国法医学杂志》 CSCD 2005年第2期65-67,共3页 Chinese Journal of Forensic Medicine
基金 上海市科技基金资助(03-JG05018)
关键词 法医物证学 胶带 汗潜指印 DNA分型 STR分型 Forensic physical evidence Adhesive tape Sweat latent fingerprint DNA genotype STR genotype
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参考文献7

  • 1郑秀芬.法医DNA分析[M].北京:中国人民公安大学出版社,2002.37-59.
  • 2张晓梅 李德仲 张忠良.胶带粘面上汗潜手印的荧光显现[J].中国刑警学院学报,2004,(3):32-35.
  • 3马荣梁,常柏年.胶带粘面上的手印显现[J].刑事技术,2000,25(3):25-27. 被引量:16
  • 4李宏观 吴学勇 张丽梅.压敏胶带纸的剥离与指纹的显现[J].中国刑警学院学报,2004,3:36-38.
  • 5顾丽华,平原,阎建军,王德明,陈连康,顾明波,郑秀芬,周怀谷,江涛,李棨,郑会芬,唐建平,尚宝良,胡伟,张晨.汗潜指印的STR分型检测[J].中国法医学杂志,2003,18(6):329-331. 被引量:20
  • 6Schulz MM, Reichert W. Archived or directly swabbed latent fingerprints as a DNA source for STR typing [ J ]. Forensic Sci Int, 2002,127:128 - 130.
  • 7Zamir A, Springer E, Glattstein B. Fingerprint and DNA:STR typing of DNA extracted from adhesive tape after processing for fingerprints [ J ]. J Forensic Sci, 2000,45 ( 3 ):687 - 688.

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