尿液及尿斑DNA分型的研究

Study on typing of DNA extracted from urine and urine stains

目的研究尿液及尿斑的DNA提取及其检验。方法用Chelex100法及QIAampMiniKit提取尿液及尿斑样本中的DNA,进行PCR扩增及STR检验。结果新鲜的及存放时间在12h以内的尿液样本能得到较好的分型结果;存放2d左右的尿液样本有50%能检出基因型;存放7d及更长时间的尿液样本全部不能检出基因型;尿斑样本的分型成功率很低。结论较新鲜的尿液样本均能进行DNA分型,在法医检案中具有应用价值。

Objective To investigate the extraction of DNA from urine and urine stains and typing.Methods DNA was extracted from urine and urine stains with Chelex-100 extraction method and QIAamp Mini Kit.STR loci were typed after amplification by PCR procedures with Profiler Plus Kit.Results STR loci could be well typed in DNA extracted from the urine,which was fresh or preserved properly for less than 12 hours.Satisfactory DNA typing could be made in 50% of the urine preserved for two days.No typing results were obtained in the samples kept for seven days or much longer time,and typing was rarely available using urine stains.Conclusion DNA extracted from the fresh urine can be typed,which may be used in forensic identification.