期刊文献+

应用胶体金试纸条快速检测赭曲霉毒素A的研究 被引量:64

Preparation of Colloidal Gold Strip for Rapid Detection of Ochratoxin A
下载PDF
导出
摘要 本文介绍了一种快速检测赭曲霉毒素A的胶体金试纸条的研制方法,其中包括胶体金的生产、金标抗体的制备、试纸条的组装和测试等步骤。测试结果表明赭曲霉毒素A快速检测试纸条的检测限为10ng/ml,检测时间为10min,加上使用方便,经济适用,使之适合于赭曲霉毒素A现场快速检测之用。 A method for rapid detection of Ochratoxin A by colloidal gold strip was introduced. Four steps were involved in the process of the preparation of the strip, including production of colloidal gold, manufacture of immunogold, installation and analysis of the strip. With the strip, 10ng/ml of Ochratoxin A was detected in less than 10 min. Apart from very short time to get test results, the strip possessed benefits of user-friendly format and relatively inexpensive to make. So it is ideally suited for on site testing of Ochratoxin A.
出处 《食品科学》 EI CAS CSCD 北大核心 2005年第5期204-207,共4页 Food Science
基金 "十五"国家重大科技专项"食品安全关键技术"
关键词 赭曲霉毒素A 胶体金 试纸条 Ochratoxin A colloidal gold strip
  • 引文网络
  • 相关文献

参考文献17

二级参考文献34

  • 1Schlatter C, Studer-Rohr J, Rasonyi T. Carcinogenicity and kinetic aspects of ochratoxin A[J]. Food Addit Contam, 1996,13(Suppl):43-44.
  • 2Sharman M,MacDonald S. Automamated liquid chromatographic determination of ochratoxin A in cereals and animal products using immunoaffinity column clean-up [J]. J Chromatography, 1992,603:285-289.
  • 3Jornet D, Busto O, Guasch J. Solid -phase extraction applied to the determination of ochratoxin A in wines by reversed-phase High-performance liquid chromatography[J]. J Chromatography,2000,882(A):29-35.
  • 4Solfrizzo M, Avantaggiato G, Viscontl A. Use of various clean-upprocedures for the analysis of ochratoxin in cereals[J].J Chromato- graphy,1998,815(A):67-73.
  • 5魏润蕴 王秀云 胡云娟.霉变粮食中赭曲霉毒素A的测定[J].中华预防医学杂志,1984,(2):113-113.
  • 6Holmberg T, Breitholtz-Emanuelsson A, Hggblom P, et al. Penicillium verrucosum in feed of ochrotoxin A positive swine herds. Mycopathologia, 1991, 116: 169-176
  • 7Jonsson N, Pettersson H, Schnürer J. Study of the relationship between storage conditions and the growth of moulds and production of ochratoxin A in grain-preliminary results.1997. St Joseph, MI: 1997annual ASAE International meeting,1997
  • 8Tren J, Varga J, Hamari Z, et al. Immunochemical detectionof ochratoxin A in black Aspergillus strain. Mycopathologia, 1996, 134:171-176
  • 9Horie Y. Productivity of ochratoxin A of Aspergillus carbonarius in Aspergillus section Nigri. Nippon Kingakukai Kaiho, 1995, 36: 73-76
  • 10JECFA. Evaluation of certain mycotoxins in food. Fifty-sixth reportof the Joint FAO/WHO Expert Committee on Food Additives, 2001, 26-35

共引文献53

同被引文献872

引证文献64

二级引证文献463

;
使用帮助 返回顶部