摘要
目的探讨血小板衍生生长因子-BB(PDGF-BB)和表皮生长因子(EGF)对人牙周膜(PDL)细胞增殖、纤维结合蛋白(Fn)和碱性磷酸酶(APLase)活性的影响。方法体外培养人PDL细胞,与不同浓度的PDGF-BB、EGF或PDGF-BB+EGF作用,用噻唑盐(MTT)法观察PDL细胞增殖的情况,ELISA法检测Fn水平,用酶动力学方法检测ALPase的活性。结果PDGF-BB明显促进PDL细胞增殖,在1~50ng/ml和5d范围内,呈浓度-时间依赖关系,最佳效应时间和浓度为3d和10ng/ml,比对照组增加了256.1%(P<0.001),但对PDL细胞Fn水平无明显影响(P>0.05),却显著抑制PDL细胞的ALPase活性(P<0.01)。低浓度的EGF对PDL细胞的增殖没有影响(P>0.05),(10~50)ng/ml的EGF从第3天起,可促进PDL细胞的增殖,差异显著(P<0.05),但10ng/ml与50ng/ml之间无显著性差异(P>0.05),其最佳效应浓度为10ng/ml,最大效应在用药后4d,此时与对照组相比增加了124.1%,EGF可使PDL细胞Fn水平明显提高(P<0.01),但抑制ALPase的活性(P<0.001)。PDGF-BB和EGF二者联合应用,在促进增殖、提高Fn的水平和抑制ALPase活性方面均具有协同效应。结论PDGF-BB、EGF均可促进人PDL细胞的增殖、抑制PDL细胞的ALPase活性,但PDGF-BB对其Fn水平没有明显影响,而EGF可使其Fn水平明显提高,二者联合具有协同效应。
Objective To evaluate the in vitro biological effects of plat elet-derived growth factor-BB(PDGF-BB) and/or epidermal growth factor(EGF) on th e proliferation, fibronectin and alkaline phosphatase activity of human periodon tal ligament(PDL) cells. Methods PDL cells were obtained from healthy donors. C ells(6×105/200 μL) were cultured for 1,2,3,4, or 5 days in DMEM supplemented w ith 2% FBS alone(control),or containing PDGF-BB(1,5,10 or 50 ng/ml), or containi ng EGF(1,5,10 or 50 ng/ml) or containing both. Proliferation of the PDL cells wa s measured by MTT colorimeteric assay. Fibronectin was determined by ELISA. ALPa se activity was measured by enzyme kinetic methods. Data was analyzed by ANOVA. Results PDGF-BB induced proliferation of PDL in a dose-and time dependent manne r(P<0.01) with an optimal concentration of 10ng/ml, at day 3. Maximal effect on proliferation was 256.1%. PDGF-BB inhibited ALPase activity but did not affect f ibronectin synthesis. EGF up-regulated the synthesis of fibronectin and inhibite d ALPase activity. Lower concentration of EGF demonstrated no significant differ ences from control, whereas it enhanced the proliferation of PDL cells at 10~50 ng/ml, with an optimal concentration of 10ng/ml, at day 4. Maximal effect on pro liferation was 124.1%. The effect of combination of PDGF-BB with EGF was the mos t signification one (P<0.001). Conclusion PDGF-BB and/or EGF can promote the pr oliferation of PDL cells and inhibited ALPase activity. EGF up-regulated the syn thesis of fibronectin, but not the PDGF. The data suggested that these growth fa ctors might be valuable in promoting the regeneration of periodontal connective tissue.
出处
《热带医学杂志》
CAS
2005年第3期267-270,281,共5页
Journal of Tropical Medicine
基金
广州市科技局资助项目(No.1999-J-008-01)