HSV-tK与IL-2基因对涎腺多形性腺瘤细胞的杀伤作用

The Effects of HSV-tk Suicide Gene and IL-2 Gene on Pleomorphic Adenoma Cells of Salivary Gland

目的:观察腺病毒介导的单纯疱疹病毒胸苷激酶基因(HSV-tK)和白细胞介素-2基因(IL-2)联合杀伤人涎腺多形性腺瘤细胞的效果。方法:采用腺病毒介导的HSV-tK基因和IL-2基因联合转染人涎腺多形性腺瘤细胞;采用逆转录聚合酶链式反应(RT-PCR)检测转染后细胞的基因表达;采用四唑盐比色(MTT)法测定tK与IL-2基因对肿瘤细胞的杀伤作用及旁观者效应;采用光镜观察基因治疗后肿瘤细胞的形态学变化。结果:单独转染HSV-tK/GCV及IL-2基因5天后,涎腺多形性腺瘤细胞的存活率分别为37.7%和66.0%;联合转染5天后,细胞的存活率降至21.5%,与前二者相比差异有显著性(P<0.05)。结论:HSV-tK和IL-2基因联合使用对涎腺多形性腺瘤的杀伤作用高于上述基因单独使用的杀伤作用。

Objective: To study the therapeuetic effect of combined gene therapy with herpes simplex virus thymidine kinase (HSV-tK) and interleukin-2(IL-2) on pleomorphic adenoma cells of salivary gland. Methods: IL-2 and HSV-tK gene were transfected into human pleomorphic adenoma cells of salivary gland using recombinant adenovirus vector. The efficiency of transfection was checked and gene was expressed using RT-PCR methods. The cell inhibition after transfection was verified by light microscope and MTT. Results: The proliferation of pleomorphic adenomas cells transfected HSV-tk and IL-2 gene was inhibited and the cell survival rate was decreased to 37.7% and 66.0% respectively in 5 days. However, when IL-2 gene combined with HSV-tK/GCV system, the killing effects was significantly stronger (P<0.05) and the cell survival rate was decreased to 21.5%. Conclusion: Combining IL-2 gene with HSV-tK/GCV system for gene therapy in pleomorphic adenoma cells of salivary gland is a valuable method.