提高急性心肌梗死大鼠模型存活率的实验研究

Experimental study of improving survival rate of acute myocardial infarction rat model

目的:建立一种简便、轻创、存活率高的急性心肌梗死大鼠实验模型。方法:SD大鼠戊巴比妥钠复合麻醉,经口气管插管,左胸第3、4肋间开胸,结扎左冠状动脉前降支。8周后行心电图观察及血流动力学、左心室功能、病理检测。结果:本法术后24h内大鼠死亡率为11.82%;存活8周的心肌梗死者大鼠可达80.65%。与假手术组相比,心肌梗死组左室重、左室舒张末压、心率均显著升高,收缩压、舒张压、平均压、左室收缩压、左室内压最大收缩和舒张速率均显著降低(P<0.05)。结论:此法简单、有效、重复性好,对研究心肌梗死后左心室重构有重要意义。

Objective:To establish an acute myocardial infarction rat model which is easy to perform with slight damage and high survival rate.Method:Sprague-Dawley rats were anaesthetized with sodium Phenobarbital. Tracheotomy intubation through mouth and left anterior thoracotomy through 3,4 intercostal region were performed. Left anterior descending coronary artery was ligated. Then electrocardiogram change, hemodynamics and left ventricular function were observed and measured in eight weeks.Result:Rat mortality was 11.82% in 24 hours after operation. The survival rate was 80.65% beyond 8 weeks.Compared with the sham-operated group,the left ventricular(LV)weight(LVW),LV end diastolic pressure(LVEDP)and heart rate(HR)were all significantly increased,while blood pressure(BP), LV systolic pressure(LVSP), the left ventricular pressure maximal rate of rise and fall (±dp/dtmax ) and their adjustment by LVSP(±dp/dtmax/LVSP) were significantly decreased(P<0.05) in operated group. Conclusion: The model construction method is simple,effective,and repeatable,which may be important for research of left ventricular remodeling post myocardial infarction.