电针刺大鼠足三里穴对LESP血浆胃泌素和胃动素的影响

Effect of electro-acupuncture at Zusanli acupiont on LESP, plasma gastrin and motilin in rats

目的:观察电针刺足三里穴对大鼠下食管括约肌压力(LESP)的影响以及与血浆胃泌素(GAS)和胃动素(MTL)的关系.方法:将Wistar大鼠40只随机分成5组:对照组、非经非络穴组、足三里穴组、阿托品组和阿托品+足三里穴组.应用三通道微细注水式测压管和荷兰UPS-2020型食管测压系统测定大鼠LESP,同时放免法测定血浆GAS和MTL的水平.结果:电针刺大鼠足三里穴和非经非络穴及电针刺停止后,LESP均有明显升高[升高率:62.7±27.0%,131.0±36.4%(0-20 min),122.6±25.9%(21-40 min),P＜0.01],足三里穴组LESP升高明显大于非经非络穴组(2.34±0.69 kPa vs 1.94±0.35 kPa,3.308±0.88 kPa vs2.30±0.36 kPa,3.17±0.69 kPA vs 2.22±0.40 kPa,P＜0.01).大鼠阿托品ip后LESP明显下降(P＜0.01);在电针刺期间及电针刺后LESP升至正常,升高率明显低于足三里穴组(P＜0.01).电针刺大鼠足三里穴后血浆GAS,MTL水平与对照组相比明显升高(P＜0.01);非经非络穴组升高不明显(P1=0.492,P2=0.104);阿托品+足三里穴组GAS和MTL比阿托品组明显升高(P1＜0.01、P2＜0.05);GAS和MTL变化与LESP呈明显正相关(r1=0.664、r2=0.703,P＜0.01).结论:电针刺对LESP的调控作用具有一定的经络和穴位特异性;胆碱能M受体阻断可部分减弱电针刺足三里的作用,电针刺可以使胆碱能神经阻断大鼠降低的LESP恢复至正常;胃泌素和胃动素可能参与了电针刺对LESP的调控作用.

AIM: To investigate the influence of electro-acupuncturing (EA) at Zusanli acupoint on the pressure of lower esoph-ageal sphincter (LESP), and the plasma levels of gastrin (GAS) and motilin (MTL) in the rats. METHODS: Forty rats were divided into five groups: control group, non-channel non-collateral acupoint group, Zusanli acupoint group, atropine group, and atropine+Zusan/Zgroup. LESP was recorded by using three-channel perfusion manometric measurement system. Radioimmunoassay was applied to detect the plasma contents of GAS and MTL in the rats. RESULTS: LESP increased significantly under or after EA at Zusanli acupoint in both Zusanli group and non-channel non-collateral acupoint group [Increased rate: 62.7±27.0%, 131.0±36.4% (0-20 min), 122.6±25.9% (21-40 min), P<0.01 ]. Furthermore, the variation degrees of LESP in Zusanli acupoint group were significantly higher than that of the latter(2.34±0.69 kPa vs 1.94±0.35 kPa, 3.308±0.88 kPa vs 2.30±0.36 kPA, 3.17±0.69 kPA vs 2.22±0.40 kPa, P<0.01). LESP of atropine+Zusanli group decreased sig- nificantly after intraperitoneal injection of atropine, and restored to the basic level under EA or after EA, and the variation degrees were significantly lower than those of Zusanli group (P<0.01). GAS and MTL levels in plasma of Zusanli group were significantly higher than those of control group (P<0.01). No significant difference was found between non-channel non-collateral acupoint group and control group (P1 =0.492, P2 =0.104). GAS and MTL of atropine+Zusan// group increased significantly compared with atropine or control group (P1<0.01, P2<0.05). GAS and MTL levels in plasma were positively correlated with LESP (P<0.01, r1 =0.664, r2 =0.703). CONCLUSION: There might be acupoint and meridian specificity in the regulatory effect of EA on LESP. Cholin-ergic M receptor blocker partially abolishes the effect of EA on LESP, but EA can restore the decreased LESP in the rats treated with cholinergic M receptor blocker. EA at Zusanli acupoint can increase plasma GAS and MTL levels, which are positively correlated with LESP of rats, indicating that some brain-gut peptides may play a role in the effect of EA at Zusanli acupoint.