铜中毒对雏鸭免疫器官细胞凋亡影响的研究

Effect of Copper Toxicity on Lymphocyte Apoptosis of Lymphoid Organs in Ducklings

选用1日龄天府肉鸭健雏210只,随机分为3组,分别喂以对照日粮(Cu12.16mg/kg)、中毒I日粮(Cu850mg/kg)和中毒II日粮(Cu1050mg/kg)7周,以超微病理学和流式细胞术的方法观察研究了铜中毒对雏鸭免疫器官淋巴细胞凋亡的影响。电镜观察,铜中毒I组和中毒II组免疫器官淋巴细胞发生凋亡的频率明显高于对照组。发生凋亡的淋巴细胞表现为染色质凝聚、边集、细胞核呈新月形、“C”形、花瓣状、圆环状或圆斑状,并出现凋亡小体。流式细胞术测定,2个中毒组免疫器官淋巴细胞凋亡百分率也明显高于对照组。上述研究结果表明,铜中毒可以诱导淋巴细胞凋亡。

one-day-old Tianfu ducklings were divided into three groups, and fed on diets as follow: controls (Cu 12.16 mg/kg),copper toxic I (Cu 850 mg/kg) and copper toxic Ⅱ ( Cu 1 050 mg/kg), the effect of copper toxicity on lymphocytes apoptosis of lymphoid organs in duckling were studied by the methods of ultrastructural pathology and Flow Cytometry (FCM). Ultrastructurally, the mitochondria of lymphocytes of lymphoid organs were swelled, and its crista were broken or / and disappeared. The apoptotic lymphocytes showed that the chromatin become condensed and marginated, and the shape of nuclei were crescent, “C”-shaped, petal-like, or dark round. Also, apoptotic bodies were observed. Statistical analyses by FCM indicated that the percentage of apoptotic cells of the lymphoid organs is higher in copper toxic groups I and II than that in control group. The aforementioned results demonstrated that copper toxicity can induce apoptosis in lymphocytes of lymphoid organs.