猪多杀性巴氏杆菌的分离鉴定及生物学特性研究

The Isolation, Identification and Biological Characteristics Research of Swine Pasteurella multocida

用PCR方法配合生化鉴定,从有肺炎症状猪的肺脏及进行性萎缩性鼻炎(Progressive atrophic rhinitis,PAR)症状猪的鼻拭子中分离出66 株多杀性巴氏杆菌(Pasteurella multocida, Pm)。然后做了药敏试验,并用PCR方法对这66株Pm进行分型及毒素基因的检测, 用豚鼠皮肤坏死试验及小鼠致死试验对产毒素多杀性巴氏杆菌(Toxigenic Pasteurella multocida, T+Pm)进一步鉴定。结果显示PCR鉴定与生化鉴定Pm结果完全一致;PCR分型表明有46株为D型Pm,18株为A型Pm,1株为B型Pm,1株无法定型;有8株用PCR检测为T+ Pm;豚鼠皮肤坏死试验及小鼠致死试验对这8株T+Pm的进一步鉴定也表明均为产毒素菌株。所鉴定的8 株T+ Pm都为D型,都分离于有严重PAR症状的猪。

With PCR and biochemical reaction test, sixty-six strains of Pasteurella multocida(Pm) was isolated from the lung tissues of pneumonic swine or nasal swabs of swine suffering progressive atrophic rhinitis(PAR) swine. Their medicine sensitivity was then studied. PCR assay was used to type these Pm isolates, as well as test the toxA gene. Guinea pig skin test and mouse lethal test were used to study the toxigenic pasteurella multocida (T +Pm). The results showed that all PCR positive strains were coincident with the biochemical reaction criteria of Pm. PCR typing assay indicated that forty-six strains were serogroup D, eighteen strains were serogroup A, one strain was serogroup B and one strain was untypable. Eight strains were identified as T +Pm by PCR method. Guinea pig skin test and mouse lethal test also confirmed that the eight isolates were T +Pm. All the eight T +Pm strains were serogroup D, and were isolated from the swines suffering severe PAR.