摘要
目的明确碱性成纤维细胞生长因子(bFGF)对体外成骨细胞和牙周膜成纤维细胞迁移、增殖的影响,以探讨在牙种植体组织界面局部应用bFGF诱导类牙周膜形成的可行性。方法同一只SD大鼠来源的成骨细胞和牙周膜成纤维细胞经传代培养至第4代,建立体外创伤模型,分别在普通培养基和含bFGF的培养基中培养,观察细胞迁移情况,四唑盐比色实验(MTT)测定细胞的增殖速度。结果普通培养基中,成骨细胞迁移速度快于成纤维细胞。加bFGF培养基中牙周膜成纤维细胞迁移速度明显快于其他各组,同时MTT结果显示加入bFGF能明显促进两种细胞的增殖。结论bFGF能明显促进牙周膜成纤维细胞的增殖、移行。
Objective In order to determine the influence of bFGF on the biological characteristics of osteoblasts and periodontal ligament cells, the author investigate the influence of bFGF on the migration, proliferation of osteoblasts and periodontal ligament fibroblasts between osteoblasts and fibroblasts.Methods The forth generation of osteoblasts and periodontal ligament fibroblast were obtained and the wounding model in vitro of them was founded. The cells were cultured in mediums with or without bFGF. Their migration and proliferation were observed and measured.Results Osteoblasts migrated faster than fibroblasts in common culture medium without bFGF and the periodontal ligament fibroblasts in culture medium with bFGF had higher migration rate than other groups. bFGF promoted the proliferation of both two kinds of cells.Conclusion bFGF could accelerate periodontal fibroblasts′ proliferation and migration.
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2005年第3期201-203,共3页
West China Journal of Stomatology
基金
高等学校博士学科点专项科研基金资助项目(B120010610059)