不同频率电针对大鼠坐骨神经损伤后神经组织形态学与骨骼肌肌电图的影响

Effects of electroacupuncture at different frequencies on morphological changes of nervous tissues and electromyogram of skeletal muscles in the rat with injury of sciatic nerve

目的:观察不同频率电针对再生过程中神经组织形态学以及骨骼肌肌电图(EMG)的影响,寻找较适宜的电针参数。方法:构建坐骨神经截断后神经再生室,以5 Hz和100 Hz电针刺激患侧"环跳""足三里"和"三阴交",每次治疗10 min,隔日1次,每周治疗3次;并设对照组观察。各组治疗时间为20 w。以神经纤维银染以及超微结构观察电针治疗组和对照组的再生组织形态学变化;应用Biopac多导电生理仪,测定腓肠肌EMG。结果:电针刺激能明显促进坐骨神经损伤后神经组织形态及失神经支配腓肠肌功能的恢复,其中以5 Hz电针组治疗效果最佳。结论:电针是促进周围神经损伤再生的重要手段。

Objective To observe effects of electroacupuncture of different frequencies on morphological changes of nervous tissues and electromyogram (EMG) of skeletal muscles in regeneration,so as to find more proper parameters of electroacupuncture. Methods The nerve regeneration chamber was built after the sciatic nerve was transected, and acupuncture was given at 'Huantiao' (GB 30) ,' Zusanli' (ST 36) and 'Sanyinjiao' (SP 6) at the affected side with different frequencies (5 Hz, 100 Hz), 30 min each time, once every other day, 3 time each week, for 20 weeks and a control group was set up for natural recovery. The nerve fibers were stained by silver dying and ultra-structures of nerve tissues were observed. EMG of gastrocnemius was determined by Biopac physiologic apparatus. Results Electroacupuncture could promote the recovery of form of nerve tissues after the transection of sciatic nerve and functions of de-innervated muscle tissues after lesion of sciatic nerve, and the therapeutic effect of electroacupuncture at 5 Hz was the best, Conclusion Electroacupuncture is an important way of promoting the regeneration of the lessoned peripheral nerve.