摘要
收集非何杰金淋巴瘤(NHL)标本59例。用全T(UCHL一1)与全B(L26)McAb作免疫组化分型。然后应用lgH单轮和半重叠基因引物,T细胞受体β(TCR_β)基因引物进行多聚酶链反应(PCR)扩增检测克隆性基因重排。检测阳性结果:新鲜组织lgH71.4%(10./14),TCR_β83.3%(10/12)。石蜡包埋组织IgH(半重叠扩增)80%(12/15),TCR_β73.3%(11/15)。无假阳性。其中有6例有争议的疑难病例明确了诊断。结果表明PCR技术是当前最特异、敏感而快速的NHL克隆性基因重排检测方法。
n this study, specimens from 59 cases of non一Hodgkin′s lymphoma(NHL) wereimmunophenotyped with L26 and U CHL一I McAb, then investigated for gene rearrangement by PCRtechnique。 The results showed that the rates of positive amplification of clonal lgH and TCR_β generearrangement were 71.4% (10/ 14) and 83.3%(10 / 12) respectively in the fresh tissues, and the lgH(semi一nested PCR technique) and TCR_β were 80%(12 / 15) and 73.3%(11 / 15) respectively in paraf-fin embedded tissues. Definitive diagnosis was made for the 6 cases which could not be diagnosed byroutine and immunohistochemical methods,and all 6 exhibited lgH or TCR_βsingle band No caseswith pseudopositive amplification were discovered. This study suggested that the PCR technique wasthe most specific,sensitive and rapid detection method for clonal gene rearrangement in NHL。
出处
《中华病理学杂志》
CAS
CSCD
北大核心
1994年第4期207-210,共4页
Chinese Journal of Pathology
关键词
聚合酶链反应
淋巴瘤
非何杰金
Polymerase chain reaction Lymphoma, non-hodgkin′s Gene rearrangement