摘要
以滇黄精为材料,经热水提取,乙醇沉淀和二乙氨基乙基(DEAE)纤维素柱、Sepha-dex柱层析分离得到3个多糖组分(PKPI,PKPⅡ及PKPⅢ),其中滇黄精多糖I(PKPI)主要由葡萄糖组成,相对分子质量M约为8100。经酸水解、乙酰化、甲基化反应、红外吸收光谱(IR)、核磁共振氢谱(1HNMR)、气相色谱(GC)及气相色谱-质谱联用技术(GC-MS)分析证明其化学结构为以α-(1→4)葡萄糖为主链,并在6-O上有少量短支链。
Three polysaccharide components were isolated from Polygonatum kingianum Coll.et Hemsl, by hot water extraction,ethanol precipitation,and purification through DEAE-cellulose and gel permeation column chromatography. The structural feature of P. kingianum polysaccharide I (PKP I) was identified by chemical (including sugar composition analysis, methylation analysis and periodate oxidation) and spectral analyses (IR, 1H NMR and GC-MS). The results showed that PKP I was mainly composed of glucose, the main chain of polysaccharide was α -(1→ 4) linked, and mean molecular weight was 8 100.
出处
《林产化学与工业》
EI
CAS
CSCD
2005年第2期80-82,共3页
Chemistry and Industry of Forest Products
基金
武汉市青年晨光计划(20035002016-27)
关键词
滇黄精
多糖
Polygonatum kingianum Coll. et Hemsl
polysaccharides
