摘要
对不同条件下黑木耳多糖酶法提取的影响因素进行了研究,结果表明,利用酶法结合热水浸提法提取黑木耳多糖,提取率达17.2%。酶解最适作用条件为:复合酶反应的最适pH值为5,添加量为1.5%,反应100min;蛋白酶反应的最适pH值为6,添加量为2%,反应60min,80℃浸提90min。
We study the influence factors of extraction on Auricularia auricular polysaccharides in enzymatic methods under different conditions. The result is that the extractive rate is 17.2% when extracting auricularia auricular polysaccharides in enzymatic and hot water soaking method. The best conditions of enzymatic action is that the best pH of complex enzyme is 5 and the amount is 1.5% and reacting time is 100min, and the best pH of protease is 6.5 and the amount is 2% and reacting time is 60min,soaking 90min in hot water at 80℃.
出处
《食品研究与开发》
CAS
北大核心
2005年第3期89-91,共3页
Food Research and Development
基金
黑龙江省教育厅重大研究项目
项目号:10511Z006
关键词
黑木耳
多糖
蛋白酶
纤维素酶
果胶酶
提取条件
Auricularia auricular
polysaccharides
protease
cellulase
pectase
extraction