提取条件对白鲢肌动球蛋白得率及Ca^(2+)-ATPase活性的影响

Effect of Extraction Condition on the Recovery Rate and Ca^(2+)-ATPase Activity of Actomyosin in Silver Carp

本文以白鲢为原料,研究肌动球蛋白提取条件对其得率及Ca2+-ATPase活性的影响。结果表明:用水漂洗1次,低盐磷酸缓冲液清洗3次后肌动球蛋白得率及其Ca2+-ATPase活性较高;肌动球蛋白得率及其Ca2+-ATPase活性均随浸提温度的升高而下降,温度低于20℃时对Ca2+-ATPase活性的影响较小,但高于20℃时,Ca2+-ATPase活性随浸提温度的升高而迅速下降;浸提时间极显著地影响Ca2+-ATPase活性,离心速度对得率影响显著,其最佳浸提时间、离心速度分别为22h和8000r/min。在所得的适宜提取工艺条件下,肌动球蛋白得率为12.57%,Ca2+-ATPase活性可达60.62U/g。

Effect of extraction condition on the recovery rate and Ca 2+ -ATPase activity of actomyosin was investigated. The result suggested that the recovery rate and Ca 2+ -ATPase activity were high respectively after water-washing one time and buffer- washing three times. Both of them decreased with increasing extraction temperature. The activity hardly changed below 20℃, but it obviously went down while extraction temperature was over 20℃. Extraction time had significant influence on the activity, and also centrifugal velocity on the recovery rate. And the optimal time was 20 hours and centrifugal velocity 8000r/ min, respectively. Under the extraction condition that had been optimal, the recovery rate of actomyosin was 12.57% and Ca 2+ - ATPase activity reached 60.62U/g.