摘要
以人类组织相容性抗原（ＨＬＡ）的ＤＱβ片段ｃＤＮＡ作为探针，用４种限制性内切酶（ＭＳＰＩ、ＥｃｏＲＩ、ＴａｑＩ和ＰｓｔＩ）对６对单倍体相同的活体肾移植供受者进行ＤＮＡ配型。结果发现ＨＬＡ一ＤＮＡ分型比血清学配型更高频率地表现其特异性，４种酶中ＥｃｏＲＩ的分型尤与肾移植存活率相关。混合淋巴细胞培养结果也与ＥｃｏＲＩ分型相近。
estrictive Fragment Length Polymorphism(RFLP) was used to analize HLA一DNA typing for 6 pairs of donor and recipient in one haplotype identical renaI transplantation。EcoR I,Taq I,MSP I and PST I respectively as restrictive enzymes are separated by agarose electrophoresis and transferred to a nylon membrane. DP β cDNA was used as HLA cDNA probes. The results indicated that the number of DNA bands mismatched in each case was proportional to graft survival.This HLA一DNA typing seems to be usefol as a complementary tissue typing test for transplantation.
关键词
组织相容性
抗原
RFLP
HLA
DNA
DNA HLA antigens Restriction fraginent length polymornhisms
